Apolipoprotein A5 (APOA5 or APO A-V) polymorphisms have long
been reported to be associated with cardiovascular disease and plasma lipid levels.
The present study was undertaken to investigate the relationship between the
rs662799, rs3135507, and rs2075291 with biochemical parameters in the Turkish
Cypriot population. A total of 100 Turkish Cypriot volunteer subjects (53 female
and 47 male), with a mean age of 40.8, participated in the study. A basic biochemical analysis, including serum glucose, total serum cholesterol, HDL-C, LDLC, and triglycerides, was performed for each participant. Genotyping for the
APOA5 three polymorphisms was performed by polymerase chain reaction followed by restriction fragment length polymorphism analysis. Biochemical parameters except the low-density lipoprotein cholesterol (LDL-C) were all within the
normal limits. LDL-C was found to be slightly elevated in participants according to
WHO guidelines. With respect to the genotype and allele distributions of APOA5
rs662799 T[C polymorphism, TT genotypes are more frequent (62%) in the population and the frequency of T allele is 0.78. The TT genotype for APOA5
rs2075291 G\T was not observed in the study population. Ancestral GG is the only
genotype present in the study population. Minor Allele Frequency of APOA5
rs3135507 G[A variant is 0.12 for the A allele. No association between the two
studied APOA5 polymorphisms (rs662799 and rs3135507) and the biochemical
components of glucose, total cholesterol, and triglyceride were observed. On the
other hand, a strong statistical association between the high-density lipoprotein
cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) clinical
Umut Fahriog ˘lu and Mahmut C ¸ erkez Ergo ¨ren have contributed equally to this work.
& Umut Fahriog ˘lu
umutfahrioglu@gmail.com; umut.fahrioglu@neu.edu.tr
1 Faculty of Medicine, Department of Medical Biology, Near East University, Near East
Boulevard, Nicosia, Cyprus
123
Biochem Genet
https://doi.org/10.1007/s10528-017-9836-3
parameters and APOA5 rs662799 CC and rs3135507 AA genotypes was found
(p = 0.014 and p = 0.017, respectively). APOA5 polymorphisms rs662799 and
rs3135507, with the CC and the AA genotypes, respectively, are associated with
increased levels of both high-density lipoprotein cholesterol (HDL-C) and lowdensity lipoprotein cholesterol (LDL-C) in the Turkish Cypriot population.

• Biochemical Genetics